The use of lightsheet localization microscopy for clarified tissue was developed by the Research Center for Applied Sciences. By combining the advantages of localization microscopy and lightsheet microscopy, it proved possible to obtain super-resolved 3D images at sub 100 nm resolution across the cleared brain of a fly. Uncovering the Memory Mechanism In Our Brains–Developing Lightsheet Localization Microscopy for Clarified Tissue “Observing cells with a microscope allows us to solve the enigmatic biology of organisms. Therefore, by continuously improving optical imaging techniques, we can look forward to resolving unprecedented details of bio-structures, thereby discovering neuron connections in the human brain in order to unveil the mysteries of life.” –Bi-Chang Chen, Associate Research Fellow, Research Center for Applied Sciences Visualizing memory formation in brains then becomes feasible by mapping the subcellular distribution of monoamine transporter proteins in the axons of the serotonergic Dorsal Paired Medial (DPM) neurons.
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